Broadly tunable ultrafast laser for two-photon microscopy and spectroscopy

Discovery NX is an ultrafast tunable laser with performance that truly redefines possibilities for non-linear imaging.

Breakthrough power levels across a full octave spanning tuning range (660 nm to 1320 nm) and short pulses delivered right to the sample plane, enables deep in-vivo excitation of all popular fluorescent probes and calcium indicators. Extraordinary performance at 1300 nm provides standout capability for label-free Third Harmonic Generation microscopy.

A high power (3.5 W at 1040 nm), secondary output further allows simultaneous, multi-wavelength excitation of multiple fluorescent markers or photoactivation of optogenetics probes. The two outputs of the laser have phase-locked pulse trains, enabling Raman techniques such as CARS.

Total Power Control (TPC) represents a landmark advancement in the evolution of turnkey multiphoton laser sources, offering high-speed power modulation on both outputs. Pristine beam quality, power contrast, and stability can now be delivered directly into the microscope scan head so users may spend more time on acquiring quality images and less time on system setup and maintenance.

		Chameleon Discovery with Total Power Control The first laser of its type to offer built-in, fast power modulation on both outputs for a variety of multiphoton imaging requirements. > Watch our video to learn more!

Wavelength Extensions for Powerful Spectroscopy and Microscopy

The octave spanning tuning of Chameleon Discovery can be augmented by The Discovery VUE second harmonic generation, giving gap-free tuning from 330 nm to 1320 nm.

Tuning to the Mid-IR range is also possible using the Levante IR femtosecond OPO, which is pumped by the 1040 nm output from the Discovery. This gives tuning out to 5 µm, and even up to 15 µm with difference frequency generation (DFG).

		Visit the Multiphoton Excitation Microscopy Resources Page Coherent has been enlightening lifescience imaging for more than 25 years. Use the link below to learn about the Coherent products that have been making it possible for MPE users to focus on their samples instead of the laser equipment. > Visit the MPE resources page