Chameleon Family

Chameleon Family

Family of products designed to expand the frontiers of MPE solutions. Chameleon lasers feature the widest tuning range and highest power at any wavelength.


 
Coherent's Chameleon - Receives the IOP Innovation Awards in 2013.

Click to View

Video provided by the Institute of Physics (IOP).

Download GUI Updates: Chameleon and Talisker

Updated GUI software for control of Chameleon and Talisker DPSS lasers is availalbe from the Coherent GUI web page.


Download the latest release in either 32bit or 64bit versions. Future releases can be downloaded from this location as they become available.

 

GUI Interface
More Than 2000 Chameleon Lasers Shipped World Wide.

Originally launch in 2003, the Chameleon family of Ti:Sapphire lasers are specifically designed for use in multphoton excitation (MPE) microscopy.

Growth in deep tissue MPE applications, along with the superior benefits and unmatched product reliability can be directly attributed to the success of the Chameleon lasers.

To learn more about Chameleon's reliability, performance and long distance trip to Antarctica, click on the links below:

Welcome to Chameleon — Your Multiphoton Imaging Ti:Sapphire Laser

Multiphoton microscopy demands a laser that is simple to use, yet flexible enough to allow you to explore the widest range of wavelengths and fluorophores. Chameleon is a compact, single-box, ultrafast laser oscillator designed for completely hands-off operation.With automated tuning, Chameleon delivers optical pulses with GVD characteristics matched to commercial MPE microscopes covering a wavelength range of 400 nm. By incorporating our proprietary Power Pulse system, Chameleon can change color quickly (faster than 40 nm/s) while optimizing output power and pulse width automatically. And with the purpose-designed MRU air recirculator, Chameleon tunes effortlessly through atmospheric water vapor absorption lines, without the need for an external purge. Dependable as well as agile, Chameleon incorporates our patented, field-proven Kerr lens modelocking technique, which is the most straightforward, reliable method for producing ultrafast pulses. Chameleon requires only single-phase AC power, no external cooling water, and has a simple menu-driven operator interface. The result is a truly hands-off, virtually maintenance-free laser system, which allows you to put your focus exactly where it belongs—on your work.

Chameleon Compact OPO Family
Automated wavelength extension for Chameleon Ti:Sapphire lasers.
Chameleon Ultra Family
The flag ship of widely tunable, hands-free, modelocked Ti:Sapphire lasers.
Chameleon Ultra
Chameleon Vision Family
One-box laser solution with integrated precompensation.
Chameleon PreComp Accessory
Primarily designed for special microscope setups with high dispersion. 
Chameleon PreComp
 

 

Chameleon Family Specifications

 

Output Power (W)
Tuning Range (nm)

Chameleon Ultra

>2.5 at 800 nm
690 to 1020

Chameleon Ultra I

>2.9 at 800 nm
690 to 1040

Chameleon Ultra II

>3.5 at 800 nm
680 to 1080

Chameleon Vision I

>2.5 at 800 nm
690 to 1040

Chameleon Vision II

>3.0 at 800 nm
680 to 1080

Chameleon Vision S

>2.3 at 800 nm
690 to 1050

Chameleon Compact OPO

>0.55 at 1100 nm
1000 to 1600

Chameleon Compact OPO-Vis

>0.55 at 1100 nm
>0.11 at 550 nm
>1.4 at 400 nm
1000 to 1600
500 to 800
340 to 540

Chameleon PreComp

90% Transmission
Matches any Chameleon Ultra version
 

The new expanded Chameleon portfolio gives you the ultimate choice of one-box solutions for brighter images, better sample viability and deeper tissue penetration covering the broadest range of flurophores. Choose the new Chameleon Vision with integrated precompensation or one of the Chameleon Ultra one-box lasers with the choice to add precompensation or a Chameleon OPO wavelength extension accessory later on. Whatever your choice, you will get the following Chameleon advantages to achieve brightest images in MPE.

 

Less brightness at
1000 nm at 9.4 mW
Increased brightness at 1030 nm at 9.4 mWHighest brightness at longest wavelength - 1064 nm at 9.4 mW
Images courtesy of Jim Culver and Mary Dickinson, Baylor College

Brightest Images
Increasing the fluorescent signal is a key requirement for obtaining brighter images in MPE microscopy. The formula below shows two ways you can achieve brighter laser-excited images - either by increasing the average power or through decreasing the pulsewidth at the sample. More fluorescent signal means that more molecules in the sample are excited and this may decrease the sample viability through photo-toxicity. Even stained and fixed samples may photobleach when exposed to too much light for too long. Different samples behave in different ways. When linear damage (heating) is not important it is possible to increase the average power. When linear damage (heating) is dominant it may be more appropriate to decrease the pulse width at the sample and decrease the average power. With its high power and flexible pre-compensation, Chameleon gives you both choices.

photo

Shortest Pulsewidths: Microscopes and scanning units may have highly dispersive elements like acousto-optics modulators (AOM) or complex, high-index lenses. It is now possible for some complex MPE optical systems to exhibit more than 30,000 fs2 of GVD. This level of GVD will lengthen an unchirped pulse to many times the original duration by the time it reaches the sample. Using the formula above one can see that the longer pulses will decrease the fluorescent signal. This can be compensated by increasing the average power; however power on the sample in the order of higher than 100 mW may damage most samples. In these cases precompensating the pulses by adding a negative chirp is a good solution. Chameleon Vision enables you to manage your pulses and get the pulse duration that you think works best for your samples.

Longest wavelengths: Interest in using longer wavelengths has been increasing in the last few years. In addition to the availability of new fluorescent probes (like RFP, mCherry and mTomato), longer wavelengths are useful for SHG/THG microscopy. When used for standard MPE imaging they offer the advantage of increased flexibility of choices when you want to optimize fluorescent excitation, minimize damage to the sample or minimize light scattering in the sample. Chameleon Vision and Chameleon Ultra II tune up to 1080 nm to best reach the mFruit fluorescent proteins, while Chameleon OPO allows you to reach the limit of the water absorption around 1,400 to 1,500 nm and open new frontiers in SHG/THG and using newer fluorophores. The Chameleon platform gives you all the flexibility that you need now and in the future.

To learn how your experiment and imaging results could benefit from the industry’s broadest solutions portfolio for MPE, please contact our support team at 1-800-527-3786 or via email at tech.sales@coherent.com.

Chameleon. The ultimate MPE engine. Ready to image.

 

The Chameleon Advantage

The Chameleon Vision sets the benchmark for integrated precompensation. It uses a proprietary prism-based design that compensates the broadest range of GVD in a compact package. Traditional two-prism designs cannot deliver this same level of performance in such a small package size. This proprietary approach builds optimum beam pointing and stability into the design. It takes integrated precompensation to the next level offering the following advantages:

  • Highest output power: With more than 3 Watts, the Chameleon Vision offers the highest output power available from a laser with integrated precompensation.
  • Highest maximum negative dispersion: Many commercial microscopes now use acousto-optic devices introducing a considerable amount of pulse broadening. Home-built systems, designed for fast scanning for use with exotic lenses, may introduce the same effect. The new Chameleon Vision system is ideal to address these needs as its unique prism design adds up to 47,000 fs2 of negative dispersion usable to compensate your optical system. Chameleon Vision gives you dispersion compensation for the widest range of set-ups in order to deliver shorter pulses.
  • Highest dynamic range: The unique prism design enables you to vary the added negative dispersion over the full range - from maximum negative GVD down to 0 fs2 - without adding additional optics. This delivers peace of mind in flexibility for your setups.
  • Superior beam pointing: Chameleon Vision uses PermAlign™ technology (solder bonded optics) to mount all critical optical components. In combination with the unique prism design this provides unmatched beam pointing stability. This superior beam pointing stability translates into consistent images, scan after scan, sample after sample.
  • Widest tuning range: The widest tuning range of any integrated precompensation laser product providing the greatest flexibility in choice of fluorophores.
  • Fastest tuning: At 40 nm/s Chameleon has the fastest tuning speed on the market. A faster tuning range means that you can take images with different excitation wavelengths at a faster pace.
  • Lowest noise: The built-in single mode Verdi™ pump laser is key to Chameleon's reliability and low noise performance. Low noise from the excitation source means less variation in the excitation of each image pixel.
 

3D Video of MPE Mouse Brain image

800 micron deep brain img of mouse. Courtesy of Sara Crow, Drexel University


 
 
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